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Temperature behavior of glucose oxidase immobilized into surface-attached stimuli-sensitive copolymer microgel

, 
Olga Vladimirovna Efremova 1
Olga Vladimirovna Efremova
, 
Dmitry Vladimirovich Pergushov 1
Dmitry Vladimirovich Pergushov
1 Department of Chemistry, M.V. Lomonosov Moscow State University, Moscow, Russian Federation
10.1016/j.mencom.2023.06.038
Published 2023-06-13
Communication , Volume 33, Issue 4, 559-561
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Sigolaeva L. V., Efremova O. V., Pergushov D. V. Temperature behavior of glucose oxidase immobilized into surface-attached stimuli-sensitive copolymer microgel // Mendeleev Communications. 2023. Vol. 33. No. 4. pp. 559-561.
GOST all authors (up to 50) Copy
Sigolaeva L. V., Efremova O. V., Pergushov D. V. Temperature behavior of glucose oxidase immobilized into surface-attached stimuli-sensitive copolymer microgel // Mendeleev Communications. 2023. Vol. 33. No. 4. pp. 559-561.
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TY - JOUR
DO - 10.1016/j.mencom.2023.06.038
UR - https://mendcomm.colab.ws/publications/10.1016/j.mencom.2023.06.038
TI - Temperature behavior of glucose oxidase immobilized into surface-attached stimuli-sensitive copolymer microgel
T2 - Mendeleev Communications
AU - Sigolaeva, Larisa Viktorovna
AU - Efremova, Olga Vladimirovna
AU - Pergushov, Dmitry Vladimirovich
PY - 2023
DA - 2023/06/13
PB - Mendeleev Communications
SP - 559-561
IS - 4
VL - 33
ER -
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@article{2023_Sigolaeva,
author = {Larisa Viktorovna Sigolaeva and Olga Vladimirovna Efremova and Dmitry Vladimirovich Pergushov},
title = {Temperature behavior of glucose oxidase immobilized into surface-attached stimuli-sensitive copolymer microgel},
journal = {Mendeleev Communications},
year = {2023},
volume = {33},
publisher = {Mendeleev Communications},
month = {Jun},
url = {https://mendcomm.colab.ws/publications/10.1016/j.mencom.2023.06.038},
number = {4},
pages = {559--561},
doi = {10.1016/j.mencom.2023.06.038}
}
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Sigolaeva, Larisa Viktorovna, et al. “Temperature behavior of glucose oxidase immobilized into surface-attached stimuli-sensitive copolymer microgel.” Mendeleev Communications, vol. 33, no. 4, Jun. 2023, pp. 559-561. https://mendcomm.colab.ws/publications/10.1016/j.mencom.2023.06.038.

Keywords

enzymatic activity regulation
enzyme immobilization
glucose oxidase.
microgel
poly(N-isopropylacrylamide-co-N-(3-aminopropyl)methacrylamide)
stimuli-sensitivity

Abstract

Electrochemical responses of glucose oxidase loaded (via electrostatic immobilization) into a surface-attached pH- and temperature-sensitive copolymer microgel were examined. The observed temperature behavior of the immobilized enzyme provides evidence that such systems enable pH-dependent regulation of activity of glucose oxidase by a (repeated) temperature cycling, which reversibly transforms the polymeric (microgel) matrix from the swollen state to the collapsed one.

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