Keywords
adsorption
enzyme immobilization
poly(ionic liquid)
polyelectrolyte
polymer–enzyme interaction
thin films
Abstract
Polymer–enzyme films are prepared via sequential adsorption of an imidazolium-based poly(ionic liquid), poly(1-ethyl-3-vinylimidazolium bromide), and an enzyme, glucose oxidase. Their formation and stability toward disassembly in salt-containing aqueous media is examined by means of quartz crystal microbalance with dissipation monitoring and supported by electrochemical measurements of the enzymatic responses. A considerable fraction of the enzyme remains bound to the polymer even at high ionic strength of the surrounding solution, thus indicating remarkable stability of the polymer–enzyme films.
Funders
Russian Science Foundation
25-23-00125
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