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Immobilization of acidic polysaccharides on Sepharose for the isolation of specific antibodies

Alexander Borisovich Tuzikov 1
Alexander Borisovich Tuzikov
, 
Polina Sergeevna Obukhova 1
Polina Sergeevna Obukhova
, 
Svetlana Vladimirovna Tsygankova 1
Svetlana Vladimirovna Tsygankova
, 
Nadezhda Vladimirovna Shilova 1
Nadezhda Vladimirovna Shilova
, 
Elena A Gordeeva 1
Elena A Gordeeva
, 
Victoria V Golovchenko 2
Victoria V Golovchenko
, 
Olga A Patova 2
Olga A Patova
, 
2 Institute of Physiology, Komi Science Center, Ural Branch of the Russian Academy of Sciences, 167982 Syktyvkar, Russian Federation
10.71267/mencom.8024
Published 28 July 2026 , received 24 February 2026
Communication , Volume 36, Issue 5, 542-544
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Tuzikov A. B. et al. Immobilization of acidic polysaccharides on Sepharose for the isolation of specific antibodies // Mendeleev Communications. 2026. Vol. 36. No. 5. pp. 542-544.
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Tuzikov A. B., Obukhova P. S., Tsygankova S. V., Shilova N. V., Gordeeva E. A., Golovchenko V. V., Patova O. A., Bovin N. V. Immobilization of acidic polysaccharides on Sepharose for the isolation of specific antibodies // Mendeleev Communications. 2026. Vol. 36. No. 5. pp. 542-544.
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TY - JOUR
DO - 10.71267/mencom.8024
UR - https://mendcomm.colab.ws/publications/10.71267/mencom.8024
TI - Immobilization of acidic polysaccharides on Sepharose for the isolation of specific antibodies
T2 - Mendeleev Communications
AU - Tuzikov, Alexander Borisovich
AU - Obukhova, Polina Sergeevna
AU - Tsygankova, Svetlana Vladimirovna
AU - Shilova, Nadezhda Vladimirovna
AU - Gordeeva, Elena A
AU - Golovchenko, Victoria V
AU - Patova, Olga A
AU - Bovin, Nicolai Vladimirovich
PY - 2026
DA - 2026/07/28
PB - Mendeleev Communications
SP - 542-544
IS - 5
VL - 36
ER -
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@article{2026_Tuzikov,
author = {Alexander Borisovich Tuzikov and Polina Sergeevna Obukhova and Svetlana Vladimirovna Tsygankova and Nadezhda Vladimirovna Shilova and Elena A Gordeeva and Victoria V Golovchenko and Olga A Patova and Nicolai Vladimirovich Bovin},
title = {Immobilization of acidic polysaccharides on Sepharose for the isolation of specific antibodies},
journal = {Mendeleev Communications},
year = {2026},
volume = {36},
publisher = {Mendeleev Communications},
month = {Jul},
url = {https://mendcomm.colab.ws/publications/10.71267/mencom.8024},
number = {5},
pages = {542--544},
doi = {10.71267/mencom.8024}
}
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Tuzikov, Alexander Borisovich, et al. “Immobilization of acidic polysaccharides on Sepharose for the isolation of specific antibodies.” Mendeleev Communications, vol. 36, no. 5, Jul. 2026, pp. 542-544. https://mendcomm.colab.ws/publications/10.71267/mencom.8024.
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Keywords

affinity chromatography
antibodies
enzyme immunoassay
epitope specificity
immobilization
plant polysaccharides

Abstract

Affinity adsorbents were obtained by the quantitative immobilization of acidic polysaccharides onto Amino Sepharose. For this purpose, a fraction of the carboxy groups of the polysaccharides was converted into activated esters. Polysaccharides with a high uronic acid content (as sodium salts) are insoluble in DMSO; therefore, they were converted into soluble N,N-diisopropyl-N-ethylammonium salts and so, suitable for the immobilization reaction. Using the resulting adsorbent, anti-polysaccharide antibodies directed against plant polysaccharides were isolated from a human immunoglobulin preparation; the specificity of the isolated antibodies suggests an ability to recognize a conformational epitope within the polysaccharide.

Funders

Russian Science Foundation
25-65-00045

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