Keywords
adsorption
biocatalyst
enzyme immobilization
halloysite
thermal stability
β-galactosidase
Abstract
β-Galactosidase from the fungus Aspergillus oryzae was immobilized on halloysite nanotubes of various origins and internal diameters. Enzyme adsorption was found to reach 1.3 mg m−2 for nanotubes with an internal diameter of 50 nm, but it was half that for nanotubes with a smaller diameter (12.5 nm) owing to the poor accessibility of the nanotube’s internal surface for the adsorption of large protein molecules (Mr of ~110 kDa), regardless of the support acidity. β-Galactosidase layers immobilized on the halloysite surface retain 10–27% of activity and are 3–6 times more stable than the native enzyme, with the best results associated with protein molecules encapsulated inside the pores.
Funders
Ministry of Education and Science of the Russian Federation
AAAA-A21-121011990019-4
References
1.
Joussein E., Petit S., Churchman J., Theng B., Righi D., Delvaux B.
Clay Minerals,
2006
2.
Sánchez-Fernández J.A., Cué-Sampedro R., Saade H.
2026
3.
Fahimizadeh M., Wong L.W., Baifa Z., Sadjadi S., Auckloo S.A., Palaniandy K., Pasbakhsh P., Tan J.B., Singh R.K., Yuan P.
Applied Clay Science,
2024
4.
Atyaksheva L.F., Kasyanov I.A.
Petroleum Chemistry,
2021
5.
Zhai R., Zhang B., Wan Y., Li C., Wang J., Liu J.
Chemical Engineering Journal,
2013
6.
Yao J., Wang Q., Wang Y., Zhang Y., Zhang B., Zhang H.
Desalination and Water Treatment,
2014
7.
Wang Z., Ren D., Cheng Y., Zhang X., Zhang S., Chen W.
Heliyon,
2022
8.
Fan X., Hu M., Li S., Zhai Q., Wang F., Jiang Y.
Applied Clay Science,
2018
9.
Sillu D., Agnihotri S.
ACS Sustainable Chemistry and Engineering,
2019
10.
Balci E., Rosales E., Pazos M., Sofuoglu A., Sanromán M.A.
Environmental Technology and Innovation,
2023
11.
Pandey G., Tharmavaram M., Khatri N., Rawtani D.
Applied Clay Science,
2023
12.
Tizchang S., Khiabani M.S., Mokarram R.R., Hamishehkar H., Mohammadi N.S., Chisti Y.
Biochimica et Biophysica Acta - General Subjects,
2021
13.
Sun X., Zhang Y., Shen H., Jia N.
Electrochimica Acta,
2010
14.
Zhang Y., Cao H., Fei W., Cui D., Jia N.
Sensors and Actuators, B: Chemical,
2012
15.
Decarli N.O., Zapp E., de Souza B.S., Santana E.R., Winiarski J.P., Vieira I.C.
Biochemical Engineering Journal,
2022
16.
Recent innovations in immobilization of β-galactosidases for industrial and therapeutic applications
Duan F., Sun T., Zhang J., Wang K., Wen Y., Lu L.
Biotechnology Advances,
2022
17.
Atyaksheva L.F., Kostyukov I.A., Fastov S.A., Fedosov D.A., Shutkina O.V.
Russian Journal of Physical Chemistry A,
2024
18.
Atyaksheva L.F., Dobryakova I.V., Ivanova I.I., Knyazeva E.E., Ovsyannikov R.A., Chukhrai E.S.
Russian Journal of Physical Chemistry A,
2012
19.
Zhai R., Zhang B., Liu L., Xie Y., Zhang H., Liu J.
Catalysis Communications,
2010
20.
Tully J., Yendluri R., Lvov Y.
Biomacromolecules,
2016
21.
Maksimainen M.M., Lampio A., Mertanen M., Turunen O., Rouvinen J.
International Journal of Biological Macromolecules,
2013
22.
Andrade J.D., Hlady V., Wei A.P.
Pure and Applied Chemistry,
1992
23.
24.
Monteiro R.R., de Oliveira A.L., de Menezes F.L., de Souza M.C., Fechine P.B., dos Santos J.C.
Applied Clay Science,
2022
25.
Duce C., Della Porta V., Bramanti E., Campanella B., Spepi A., Tiné M.R.
Nanotechnology,
2016
26.
Bugatti V., Sorrentino A., Gorrasi G.
European Polymer Journal,
2017
27.
Pietraszek A., Karewicz A., Widnic M., Lachowicz D., Gajewska M., Bernasik A., Nowakowska M.
Colloids and Surfaces B: Biointerfaces,
2019