Home / Articles / Impact of base matrix types on the properties of affinity chromatography resins with ligands based on staphylococcal protein A B-domain multimers

Impact of base matrix types on the properties of affinity chromatography resins with ligands based on staphylococcal protein A B-domain multimers

Milena V Baskova 1, 2
Milena V Baskova
, 
Mikhail Nikolaevich Tereshin 1
Mikhail Nikolaevich Tereshin
, 
Kristina P Telepenina 1
Kristina P Telepenina
, 
Natalia Sergeevna Shoshina 1
Natalia Sergeevna Shoshina
, 
Alina Mikhailovna Komyakova 1, 2
Alina Mikhailovna Komyakova
, 
Barbara Zlatkovna Eletskaya 3
Barbara Zlatkovna Eletskaya
, 
Maria Yanovna Berzina 1, 3
Maria Yanovna Berzina
, 
Larisa Nikolaevna Ikryannikova 1
Larisa Nikolaevna Ikryannikova
, 
Vasily Nikolaevich Stepanenko 1, 2
Vasily Nikolaevich Stepanenko
, 
Tatiana Dmitrievna Melikhova 1, 3
Tatiana Dmitrievna Melikhova
10.71267/mencom.7973
Published 4 June 2026 , received 21 November 2025
Communication , Volume 36, Issue 4, 404-406
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Baskova M. V. et al. Impact of base matrix types on the properties of affinity chromatography resins with ligands based on staphylococcal protein A B-domain multimers // Mendeleev Communications. 2026. Vol. 36. No. 4. pp. 404-406.
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Baskova M. V., Tereshin M. N., Telepenina K. P., Shoshina N. S., Komyakova A. M., Eletskaya B. Z., Berzina M. Y., Ikryannikova L. N., Stepanenko V. N., Melikhova T. D. Impact of base matrix types on the properties of affinity chromatography resins with ligands based on staphylococcal protein A B-domain multimers // Mendeleev Communications. 2026. Vol. 36. No. 4. pp. 404-406.
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TY - JOUR
DO - 10.71267/mencom.7973
UR - https://mendcomm.colab.ws/publications/10.71267/mencom.7973
TI - Impact of base matrix types on the properties of affinity chromatography resins with ligands based on staphylococcal protein A B-domain multimers
T2 - Mendeleev Communications
AU - Baskova, Milena V
AU - Tereshin, Mikhail Nikolaevich
AU - Telepenina, Kristina P
AU - Shoshina, Natalia Sergeevna
AU - Komyakova, Alina Mikhailovna
AU - Eletskaya, Barbara Zlatkovna
AU - Berzina, Maria Yanovna
AU - Ikryannikova, Larisa Nikolaevna
AU - Stepanenko, Vasily Nikolaevich
AU - Melikhova, Tatiana Dmitrievna
PY - 2026
DA - 2026/06/04
PB - Mendeleev Communications
SP - 404-406
IS - 4
VL - 36
ER -
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@article{2026_Baskova,
author = {Milena V Baskova and Mikhail Nikolaevich Tereshin and Kristina P Telepenina and Natalia Sergeevna Shoshina and Alina Mikhailovna Komyakova and Barbara Zlatkovna Eletskaya and Maria Yanovna Berzina and Larisa Nikolaevna Ikryannikova and Vasily Nikolaevich Stepanenko and Tatiana Dmitrievna Melikhova},
title = {Impact of base matrix types on the properties of affinity chromatography resins with ligands based on staphylococcal protein A B-domain multimers},
journal = {Mendeleev Communications},
year = {2026},
volume = {36},
publisher = {Mendeleev Communications},
month = {Jun},
url = {https://mendcomm.colab.ws/publications/10.71267/mencom.7973},
number = {4},
pages = {404--406},
doi = {10.71267/mencom.7973}
}
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Baskova, Milena V., et al. “Impact of base matrix types on the properties of affinity chromatography resins with ligands based on staphylococcal protein A B-domain multimers.” Mendeleev Communications, vol. 36, no. 4, Jun. 2026, pp. 404-406. https://mendcomm.colab.ws/publications/10.71267/mencom.7973.
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Keywords

Staphylococcus aureus protein A
affinity chromatography
agarose matrix
B-domains
domain oligomerization, polymethacrylate beads
IgG binding domains B
SpA

Abstract

New chromatography resins with ligands based on oligomers of the B-domain of Staphylococcus aureus protein A were developed and characterized. Resin performance, particularly dynamic binding capacity, improved as the ligand length increased from dimer to tetramer when immobilized on a polymethacrylate surface, whereas the opposite effect was observed when using cross-linked agarose as a matrix. It was found that particle size uniformity and pore volume can play a significant role and their careful control is necessary when selecting a matrix.

References

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Engineering Staphylococcal Protein A for high-throughput affinity purification of monoclonal antibodies
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Tereshin M.N., Melikhova T.D., Eletskaya B.Z., Ksenofontova O.B., Pantyushenko P.V., Berzina M.Y., Ivanov I., Myagkikh I.V., Stepanenko V.N.
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