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Comparison of nonstoichiometric and Mg-containing calcium-deficient hydroxyapatites obtained by enzymatic synthesis

Georgy Sergeyevich Mikhaylov 1
Georgy Sergeyevich Mikhaylov
, 
Irina I. Gerasimova 1
Irina I. Gerasimova
, 
Daria-Mariia V. Ratova 1
Daria-Mariia V. Ratova
, 
Ivan Vladimirovich Mikheev 1
Ivan Vladimirovich Mikheev
, 
Olga I. Klein 2
Olga I. Klein
, 
Maria Grigor'evna Chernysheva 1
Maria Grigor'evna Chernysheva
, 
Gennadii Aleksandrovich Badun 1
Gennadii Aleksandrovich Badun
1 Department of Chemistry, M. V. Lomonosov Moscow State University, 119991 Moscow, Russian Federation.
2 Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, 119071 Moscow, Russian Federation
10.71267/mencom.7901
Published 2026-02-03
Communication , Volume 36, Issue 2, 232-234
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Mikhaylov G. S. et al. Comparison of nonstoichiometric and Mg-containing calcium-deficient hydroxyapatites obtained by enzymatic synthesis // Mendeleev Communications. 2026. Vol. 36. No. 2. pp. 232-234.
GOST all authors (up to 50) Copy
Mikhaylov G. S., Gerasimova I. I., Ratova D. V., Mikheev I. V., Klein O. I., Chernysheva M. G., Badun G. A. Comparison of nonstoichiometric and Mg-containing calcium-deficient hydroxyapatites obtained by enzymatic synthesis // Mendeleev Communications. 2026. Vol. 36. No. 2. pp. 232-234.
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TY - JOUR
DO - 10.71267/mencom.7901
UR - https://mendcomm.colab.ws/publications/10.71267/mencom.7901
TI - Comparison of nonstoichiometric and Mg-containing calcium-deficient hydroxyapatites obtained by enzymatic synthesis
T2 - Mendeleev Communications
AU - Mikhaylov, Georgy Sergeyevich
AU - Gerasimova, Irina I.
AU - Ratova, Daria-Mariia V.
AU - Mikheev, Ivan Vladimirovich
AU - Klein, Olga I.
AU - Chernysheva, Maria Grigor'evna
AU - Badun, Gennadii Aleksandrovich
PY - 2026
DA - 2026/02/03
PB - Mendeleev Communications
SP - 232-234
IS - 2
VL - 36
ER -
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@article{2026_Mikhaylov,
author = {Georgy Sergeyevich Mikhaylov and Irina I. Gerasimova and Daria-Mariia V. Ratova and Ivan Vladimirovich Mikheev and Olga I. Klein and Maria Grigor'evna Chernysheva and Gennadii Aleksandrovich Badun},
title = {Comparison of nonstoichiometric and Mg-containing calcium-deficient hydroxyapatites obtained by enzymatic synthesis},
journal = {Mendeleev Communications},
year = {2026},
volume = {36},
publisher = {Mendeleev Communications},
month = {Feb},
url = {https://mendcomm.colab.ws/publications/10.71267/mencom.7901},
number = {2},
pages = {232--234},
doi = {10.71267/mencom.7901}
}
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Cite this
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Mikhaylov, Georgy Sergeyevich, et al. “Comparison of nonstoichiometric and Mg-containing calcium-deficient hydroxyapatites obtained by enzymatic synthesis.” Mendeleev Communications, vol. 36, no. 2, Feb. 2026, pp. 232-234. https://mendcomm.colab.ws/publications/10.71267/mencom.7901.

Keywords

antimicrobial activity
enzymatic hydroxyapatite
FTIR
ICP-AES
Mg-substituted hydroxyapatite

Abstract

Enzymatic synthesis of calcium phosphate in Tris buffer with MgCl2 results in a two-fold increase in product yield compared to synthesis in water. Although some calcium positions are substituted with magnesium, both samples are classified as depleted hydroxyapatite. The Mg-containing sample exhibits higher aggregation capacity at high concentrations, resulting in reduced antimicrobial activity against gram-positive Bacillus strains.

Funders

Ministry of Education and Science of the Russian Federation
122012600116-4

References

1.
Bioactive Calcium Phosphate-Based Composites for Bone Regeneration
Tavoni M., Dapporto M., Tampieri A., Sprio S.
Journal of Composites Science, 2021
2.
Calcium Phosphates: A Key to Next‐Generation In Vitro Bone Modeling
Pandit A., Indurkar A., Locs J., Haugen H.J., Loca D.
Advanced healthcare materials, 2024
3.
Calcium Phosphate-Based Biomaterials for Bone Repair
Hou X., Zhang L., Zhou Z., Luo X., Wang T., Zhao X., Lu B., Chen F., Zheng L.
Journal of Functional Biomaterials, 2022
4.
The role of calcium phosphate surface structure in osteogenesis and the mechanisms involved
Xiao D., Zhang J., Zhang C., Barbieri D., Yuan H., Moroni L., Feng G.
Acta Biomaterialia, 2020
5.
Calcium phosphate-bearing matrices induce osteogenic differentiation of stem cells through adenosine signaling
Shih Y.V., Hwang Y., Phadke A., Kang H., Hwang N.S., Caro E.J., Nguyen S., Siu M., Theodorakis E.A., Gianneschi N.C., Vecchio K.S., Chien S., Lee O.K., Varghese S.
Proceedings of the National Academy of Sciences of the United States of America, 2014
8.
Magnetic bio-nanocomposite catalysts of CoFe2O4/hydroxyapatite-lipase for enantioselective synthesis provide a framework for enzyme recovery and reuse
Saire-Saire S., Garcia-Segura S., Luyo C., Andrade L.H., Alarcon H.
International Journal of Biological Macromolecules, 2020
9.
Hydroxyapatite: an Eco‐Friendly Material for Enzyme Immobilization
Gelati L., Rabufetti M., Benaglia M., Campisi S., Gervasini A., Speranza G., Morelli C.F.
ChemPlusChem, 2024
10.
A review on the synthesis and properties of hydroxyapatite for biomedical applications
DileepKumar V.G., Sridhar M.S., Aramwit P., Krut’ko V.K., Musskaya O.N., Glazov I.E., Reddy N.
Journal of Biomaterials Science, Polymer Edition, 2021
11.
Nouri S., Roghanian R., Emtiazi G.
Iranian Journal of Medical Microbiology, 2021
13.
Enzymatic synthesis of calcium phosphates: A review
Kharissova O.V., Nikolaev A.L., Kharisov B.I., Dorozhkin S.V., López I., Méndez Y.P., de la Fuente I.G.
Nano-Structures and Nano-Objects, 2024
14.
In-depth review of synthesis of hydroxyapatite biomaterials from natural resources and chemical regents for biomedical applications
Etinosa P.O., Osuchukwu O.A., Anisiji E.O., Lawal M.Y., Mohammed S.A., Isaac I.O., Oni P.G., Aderibigbe V.D., Aina T., Oyebode D., Nwigbo S.C.
Arabian Journal of Chemistry, 2024
15.
Sustainable synthesis of marine-derived hydroxyapatite for biomedical applications: a systematic review on extraction methods and bioactivity
Patil S., Thanikodi S., Palaniyappan S., Giri J., Hourani A.O., Azizi M.
Advanced Manufacturing: Polymer and Composites Science, 2025
16.
Synthesis method of hydroxyapatite: A review
Mohd Pu'ad N.A., Abdul Haq R.H., Mohd Noh H., Abdullah H.Z., Idris M.I., Lee T.C.
Materials Today: Proceedings, 2020
17.
Golubchikov D.O., Safronova T.V., Podlyagin V.A., Shatalova T.B., Kolesnik I.V., Putlayev V.I.
Mendeleev Communications, 2024
18.
Unraveling the role of E. coli and calf intestinal alkaline phosphatase in calcium phosphate synthesis
Chernysheva M.G., Mikhailov G.S., Ratova D.V., Mikheev I.V., Badun G.A., Nikolaev A.L.
Enzyme and Microbial Technology, 2025
19.
Synthesis and characterisation of magnesium-wrapped hydroxyapatite nanomaterials for biomedical applications
Sebastiammal S., Fathima A.S., Al-Ghanim K.A., Nicoletti M., Baskar G., Iyyappan J., Govindarajan M.
Surfaces and Interfaces, 2024
22.
Syntheses of hydroxyapatite from natural sources
Mohd Pu'ad N.A., Koshy P., Abdullah H.Z., Idris M.I., Lee T.C.
Heliyon, 2019