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In-gel detection of His-tagged proteins with 4-methoxy-1,8-naphthalimide based fluorescent probe

, 
Anna V Vlaskina 2
Anna V Vlaskina
, 
Daria E Tyupakova 1, 3
Daria E Tyupakova
, 
Sergey V Kutyakov 4
Sergey V Kutyakov
, 
Yulia K Agapova 2
Yulia K Agapova
, 
Dmitryi E Petrenko 2
Dmitryi E Petrenko
, 
Pavel Alexandrovich Panchenko 1
Pavel Alexandrovich Panchenko
, 
Olga Anatol'evna Fedorova 1
Olga Anatol'evna Fedorova
10.71267/mencom.7775
Published 2025-10-21
Communication , Volume 35, Issue 6, 681-683
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Pavlova M. A. et al. In-gel detection of His-tagged proteins with 4-methoxy-1,8-naphthalimide based fluorescent probe // Mendeleev Communications. 2025. Vol. 35. No. 6. pp. 681-683.
GOST all authors (up to 50) Copy
Pavlova M. A., Vlaskina A. V., Tyupakova D. E., Kutyakov S. V., Agapova Y. K., Petrenko D. E., Panchenko P. A., Fedorova O. A. In-gel detection of His-tagged proteins with 4-methoxy-1,8-naphthalimide based fluorescent probe // Mendeleev Communications. 2025. Vol. 35. No. 6. pp. 681-683.
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TY - JOUR
DO - 10.71267/mencom.7775
UR - https://mendcomm.colab.ws/publications/10.71267/mencom.7775
TI - In-gel detection of His-tagged proteins with 4-methoxy-1,8-naphthalimide based fluorescent probe
T2 - Mendeleev Communications
AU - Pavlova, Marina Alexandrovna
AU - Vlaskina, Anna V
AU - Tyupakova, Daria E
AU - Kutyakov, Sergey V
AU - Agapova, Yulia K
AU - Petrenko, Dmitryi E
AU - Panchenko, Pavel Alexandrovich
AU - Fedorova, Olga Anatol'evna
PY - 2025
DA - 2025/10/21
PB - Mendeleev Communications
SP - 681-683
IS - 6
VL - 35
ER -
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@article{2025_Pavlova,
author = {Marina Alexandrovna Pavlova and Anna V Vlaskina and Daria E Tyupakova and Sergey V Kutyakov and Yulia K Agapova and Dmitryi E Petrenko and Pavel Alexandrovich Panchenko and Olga Anatol'evna Fedorova},
title = {In-gel detection of His-tagged proteins with 4-methoxy-1,8-naphthalimide based fluorescent probe},
journal = {Mendeleev Communications},
year = {2025},
volume = {35},
publisher = {Mendeleev Communications},
month = {Oct},
url = {https://mendcomm.colab.ws/publications/10.71267/mencom.7775},
number = {6},
pages = {681--683},
doi = {10.71267/mencom.7775}
}
MLA
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Pavlova, Marina Alexandrovna, et al. “In-gel detection of His-tagged proteins with 4-methoxy-1,8-naphthalimide based fluorescent probe.” Mendeleev Communications, vol. 35, no. 6, Oct. 2025, pp. 681-683. https://mendcomm.colab.ws/publications/10.71267/mencom.7775.

Keywords

1
8-naphthalimide
His-tag recognition
histidine
immunoblotting.
lysine
nitrilotriacetic acid
protein labeling
UV transilluminator

Abstract

A new derivative of 4-methoxy-1,8-naphthalimide containing a chelating motif of lysine-derived mono-N-nitrilotriacetic acid effectively coordinates with Ni2+ in a HEPES-buffer solution resulting in the formation of 1 : 1 ligand-metal complex. This demonstrates the possibility to stain Hislabeled proteins on a gel after electrophoresis to make the analysis and purification of proteins more convenient.

Funders

Russian Science Foundation
24-73-00359