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SEM and SAXS characterization of recombinant lipase immobilized by adsorption on the surface of cellular carbon foam

Galina Artem'evna Kovalenko 1
Galina Artem'evna Kovalenko
, 
Larisa V Perminova 1
Larisa V Perminova
, 
Alexandra N Serkova 1
Alexandra N Serkova
, 
Aleksey Nikolaevich Salanov 1
Aleksey Nikolaevich Salanov
, 
Yurii Vasilevich Larichev 1
Yurii Vasilevich Larichev
1 G. K. Boreskov Institute of Catalysis, Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, Russian Federation
10.71267/mencom.7757
Published 2025-07-17
Communication , Volume 35, Issue 5, 592-594
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Kovalenko G. A. et al. SEM and SAXS characterization of recombinant lipase immobilized by adsorption on the surface of cellular carbon foam // Mendeleev Communications. 2025. Vol. 35. No. 5. pp. 592-594.
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Kovalenko G. A., Perminova L. V., Serkova A. N., Salanov A. N., Larichev Y. V. SEM and SAXS characterization of recombinant lipase immobilized by adsorption on the surface of cellular carbon foam // Mendeleev Communications. 2025. Vol. 35. No. 5. pp. 592-594.
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TY - JOUR
DO - 10.71267/mencom.7757
UR - https://mendcomm.colab.ws/publications/10.71267/mencom.7757
TI - SEM and SAXS characterization of recombinant lipase immobilized by adsorption on the surface of cellular carbon foam
T2 - Mendeleev Communications
AU - Kovalenko, Galina Artem'evna
AU - Perminova, Larisa V
AU - Serkova, Alexandra N
AU - Salanov, Aleksey Nikolaevich
AU - Larichev, Yurii Vasilevich
PY - 2025
DA - 2025/07/17
PB - Mendeleev Communications
SP - 592-594
IS - 5
VL - 35
ER -
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@article{2025_Kovalenko,
author = {Galina Artem'evna Kovalenko and Larisa V Perminova and Alexandra N Serkova and Aleksey Nikolaevich Salanov and Yurii Vasilevich Larichev},
title = {SEM and SAXS characterization of recombinant lipase immobilized by adsorption on the surface of cellular carbon foam},
journal = {Mendeleev Communications},
year = {2025},
volume = {35},
publisher = {Mendeleev Communications},
month = {Jul},
url = {https://mendcomm.colab.ws/publications/10.71267/mencom.7757},
number = {5},
pages = {592--594},
doi = {10.71267/mencom.7757}
}
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Kovalenko, Galina Artem'evna, et al. “SEM and SAXS characterization of recombinant lipase immobilized by adsorption on the surface of cellular carbon foam.” Mendeleev Communications, vol. 35, no. 5, Jul. 2025, pp. 592-594. https://mendcomm.colab.ws/publications/10.71267/mencom.7757.

Keywords

adsorption.
cellular carbon foam
recombinant lipase
scanning electron microscopy
surface morphology

Abstract

Using modern scanning electron microscopy (SEM), a research was conducted on the supramolecular aggregation of protein globules of recombinant Thermomyces lanuginosus lipase (TLL) adsorbed on the surface of cellular carbon foam (CCF) by the two methods of physical adsorption: spontaneous and forced (impregnation-drying). After the spontaneous adsorption, more than half of the entire surface of CCF was uniformly occupied by 10-50 nm nanoparticles, which corresponded in size to the monomers and dimers of TLL globules. After the forced adsorption, protein films were formed between and around pyrolytic carbon deposits protruding beyond these films.

Funders

Ministry of Science and Higher Education of the Russian Federation within the governmental order for Boreskov Institute of Catalysis
Project FWUR-2024-0034