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Employing gold nanoclusters for analysis of pectinase activity

Sophia Yaroslavovna Sakhina 1
Sophia Yaroslavovna Sakhina
,  ,  , 
Irina Dmitrievna Grozdova 1
Irina Dmitrievna Grozdova
, 
Margarita V. Semenova 1
Margarita V. Semenova
, 
Elena Vadimovna Kudryashova 1
Elena Vadimovna Kudryashova
,  , 
Nikolay Sergeevich Melik-Nubarov 1
Nikolay Sergeevich Melik-Nubarov
2 A. N. Belozersky Research Institute of Physico-Chemical Biology, M. V. Lomonosov Moscow State University, 119992 Moscow, Russian Federation
10.71267/mencom.7686
Published 2025-05-21
Communication , Volume 35, Issue 4, 478-480
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Sakhina S. Y. et al. Employing gold nanoclusters for analysis of pectinase activity // Mendeleev Communications. 2025. Vol. 35. No. 4. pp. 478-480.
GOST all authors (up to 50) Copy
Sakhina S. Y., Iakimov N. P., Fomin E. O., Grozdova I. D., Semenova M. V., Kudryashova E. V., Orlov V. N., Melik-Nubarov N. S. Employing gold nanoclusters for analysis of pectinase activity // Mendeleev Communications. 2025. Vol. 35. No. 4. pp. 478-480.
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TY - JOUR
DO - 10.71267/mencom.7686
UR - https://mendcomm.colab.ws/publications/10.71267/mencom.7686
TI - Employing gold nanoclusters for analysis of pectinase activity
T2 - Mendeleev Communications
AU - Sakhina, Sophia Yaroslavovna
AU - Iakimov, Nikolai Petrovich
AU - Fomin, Evgenii Olegovich
AU - Grozdova, Irina Dmitrievna
AU - Semenova, Margarita V.
AU - Kudryashova, Elena Vadimovna
AU - Orlov, Viktor Nikolaevich
AU - Melik-Nubarov, Nikolay Sergeevich
PY - 2025
DA - 2025/05/21
PB - Mendeleev Communications
SP - 478-480
IS - 4
VL - 35
ER -
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@article{2025_Sakhina,
author = {Sophia Yaroslavovna Sakhina and Nikolai Petrovich Iakimov and Evgenii Olegovich Fomin and Irina Dmitrievna Grozdova and Margarita V. Semenova and Elena Vadimovna Kudryashova and Viktor Nikolaevich Orlov and Nikolay Sergeevich Melik-Nubarov},
title = {Employing gold nanoclusters for analysis of pectinase activity},
journal = {Mendeleev Communications},
year = {2025},
volume = {35},
publisher = {Mendeleev Communications},
month = {May},
url = {https://mendcomm.colab.ws/publications/10.71267/mencom.7686},
number = {4},
pages = {478--480},
doi = {10.71267/mencom.7686}
}
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Cite this
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Sakhina, Sophia Yaroslavovna, et al. “Employing gold nanoclusters for analysis of pectinase activity.” Mendeleev Communications, vol. 35, no. 4, May. 2025, pp. 478-480. https://mendcomm.colab.ws/publications/10.71267/mencom.7686.

Keywords

3
3-ionene
aggregation-induced emission.
Au22(SG)18 glutathione-capped gold nanoclusters
pectin
pectinase activity

Abstract

A method for sensing pectinase activity was proposed based on the formation of polyelectrolyte complexes of negatively charged, glutathione-coated, fluorescent gold nanoclusters Au22(SG)18 with the polycation 3,3-ionene and the anionic polysaccharide pectin. The formation of polyelectrolyte complexes of the nanoclusters with ionene leads to an increase in fluorescence due to the aggregation-induced emission enhancement effect, while after the addition of the natural polyanion pectin, the emission decreases. Enzymatic hydrolysis of pectin by pectinase resulted in the recovery of the fluorescence of nanoclusters, which allows sensing the activity of pectinase.

Funders

Ministry of Education and Science of the Russian Federation
AAAA-A21-121011990022-4

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