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Site-specific sortase-catalyzed lipidation of proteins

Tatiana Dmitrievna Melikhova 1
Tatiana Dmitrievna Melikhova
, 
Nicolai Vladimirovich Bovin 1
Nicolai Vladimirovich Bovin
, 
Natalia Sergeevna Shoshina 2
Natalia Sergeevna Shoshina
, 
Tatiana Vladimirovna Bobik 1
Tatiana Vladimirovna Bobik
, 
Leonid A Gavrilov 1
Leonid A Gavrilov
, 
Marina Aleksandrovna Sablina 1
Marina Aleksandrovna Sablina
, 
Eugenia Markovna Rapoport 1
Eugenia Markovna Rapoport
, 
Vasily Nikolaevich Stepanenko 2
Vasily Nikolaevich Stepanenko
, 
Alexander Borisovich Tuzikov 1
Alexander Borisovich Tuzikov
2 I. M. Sechenov First Moscow State Medical University, 119991 Moscow, Russian Federation
10.71267/mencom.7613
Published 2025-02-18
Communication , Volume 35, Issue 2, 136-138
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Melikhova T. D. et al. Site-specific sortase-catalyzed lipidation of proteins // Mendeleev Communications. 2025. Vol. 35. No. 2. pp. 136-138.
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Melikhova T. D., Bovin N. V., Shoshina N. S., Bobik T. V., Gavrilov L. A., Sablina M. A., Rapoport E. M., Stepanenko V. N., Tuzikov A. B. Site-specific sortase-catalyzed lipidation of proteins // Mendeleev Communications. 2025. Vol. 35. No. 2. pp. 136-138.
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TY - JOUR
DO - 10.71267/mencom.7613
UR - https://mendcomm.colab.ws/publications/10.71267/mencom.7613
TI - Site-specific sortase-catalyzed lipidation of proteins
T2 - Mendeleev Communications
AU - Melikhova, Tatiana Dmitrievna
AU - Bovin, Nicolai Vladimirovich
AU - Shoshina, Natalia Sergeevna
AU - Bobik, Tatiana Vladimirovna
AU - Gavrilov, Leonid A
AU - Sablina, Marina Aleksandrovna
AU - Rapoport, Eugenia Markovna
AU - Stepanenko, Vasily Nikolaevich
AU - Tuzikov, Alexander Borisovich
PY - 2025
DA - 2025/02/18
PB - Mendeleev Communications
SP - 136-138
IS - 2
VL - 35
ER -
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@article{2025_Melikhova,
author = {Tatiana Dmitrievna Melikhova and Nicolai Vladimirovich Bovin and Natalia Sergeevna Shoshina and Tatiana Vladimirovna Bobik and Leonid A Gavrilov and Marina Aleksandrovna Sablina and Eugenia Markovna Rapoport and Vasily Nikolaevich Stepanenko and Alexander Borisovich Tuzikov},
title = {Site-specific sortase-catalyzed lipidation of proteins},
journal = {Mendeleev Communications},
year = {2025},
volume = {35},
publisher = {Mendeleev Communications},
month = {Feb},
url = {https://mendcomm.colab.ws/publications/10.71267/mencom.7613},
number = {2},
pages = {136--138},
doi = {10.71267/mencom.7613}
}
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Melikhova, Tatiana Dmitrievna, et al. “Site-specific sortase-catalyzed lipidation of proteins.” Mendeleev Communications, vol. 35, no. 2, Feb. 2025, pp. 136-138. https://mendcomm.colab.ws/publications/10.71267/mencom.7613.

Keywords

bioconjugation
CMG
lipid tag
mCherry
protein A
sortase А

Abstract

A methodology for the enzymatic ligation of proteins with a lipid is proposed, a feature of which is the use of a hydrophilized [thanks to a CMG(2) spacer] and therefore highly water-soluble lipid. Its H2N-Gly5 terminus and the recombinant protein amino acid sequence LPETG are substrates of the enzyme sortase A. Two lipid-ligated proteins, red fluorescent mCherry and Protein A, are shown to be inserted into the cell membrane.

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