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Efficient recombinant production of RpII, a NaV-modulating peptide from the sea anemone Heteractis magnifica

Oksana Vladimirovna Sintsova 1
Oksana Vladimirovna Sintsova
, 
Nadezhda Aleksandrovna Priymenko 1, 2
Nadezhda Aleksandrovna Priymenko
, 
Mikhail M Garbuz 3
Mikhail M Garbuz
, 
Anna Anatol'evna Klimovich 1
Anna Anatol'evna Klimovich
, 
Nikita A Shved 3, 4
Nikita A Shved
, 
Alexander Sergeevich Menshov 1
Alexander Sergeevich Menshov
, 
Stanislav Dmitrievich Anastyuk 1
Stanislav Dmitrievich Anastyuk
, 
Marina Petrovna Isaeva 1
Marina Petrovna Isaeva
, 
Irina Nikolaevna Gladkikh 1
Irina Nikolaevna Gladkikh
, 
Elena Vladimirovna Leychenko 1, 2
Elena Vladimirovna Leychenko
1 G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences, Vladivostok, Russian Federation
2 Advanced Engineering School 'Institute of Biotechnology, Bioengineering and Food Systems', Far Eastern Federal University, Vladivostok, Russian Federation
3 Department of Medical Biology and Biotechnology, Far Eastern Federal University, Vladivostok, Russian Federation
4 A.V. Zhirmunsky National Scientific Center of Marine Biology, Far Eastern Branch of the Russian Academy of Sciences, Vladivostok, Russian Federation
10.1016/j.mencom.2024.01.005
Published 2023-12-25
Communication , Volume 34, Issue 1, 15-17
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Sintsova O. V. et al. Efficient recombinant production of RpII, a NaV-modulating peptide from the sea anemone Heteractis magnifica // Mendeleev Communications. 2023. Vol. 34. No. 1. pp. 15-17.
GOST all authors (up to 50) Copy
Sintsova O. V., Priymenko N. A., Garbuz M. M., Klimovich A. A., Shved N. A., Menshov A. S., Anastyuk S. D., Isaeva M. P., Gladkikh I. N., Leychenko E. V. Efficient recombinant production of RpII, a NaV-modulating peptide from the sea anemone Heteractis magnifica // Mendeleev Communications. 2023. Vol. 34. No. 1. pp. 15-17.
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TY - JOUR
DO - 10.1016/j.mencom.2024.01.005
UR - https://mendcomm.colab.ws/publications/10.1016/j.mencom.2024.01.005
TI - Efficient recombinant production of RpII, a NaV-modulating peptide from the sea anemone Heteractis magnifica
T2 - Mendeleev Communications
AU - Sintsova, Oksana Vladimirovna
AU - Priymenko, Nadezhda Aleksandrovna
AU - Garbuz, Mikhail M
AU - Klimovich, Anna Anatol'evna
AU - Shved, Nikita A
AU - Menshov, Alexander Sergeevich
AU - Anastyuk, Stanislav Dmitrievich
AU - Isaeva, Marina Petrovna
AU - Gladkikh, Irina Nikolaevna
AU - Leychenko, Elena Vladimirovna
PY - 2023
DA - 2023/12/25
PB - Mendeleev Communications
SP - 15-17
IS - 1
VL - 34
ER -
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@article{2023_Sintsova,
author = {Oksana Vladimirovna Sintsova and Nadezhda Aleksandrovna Priymenko and Mikhail M Garbuz and Anna Anatol'evna Klimovich and Nikita A Shved and Alexander Sergeevich Menshov and Stanislav Dmitrievich Anastyuk and Marina Petrovna Isaeva and Irina Nikolaevna Gladkikh and Elena Vladimirovna Leychenko},
title = {Efficient recombinant production of RpII, a NaV-modulating peptide from the sea anemone Heteractis magnifica},
journal = {Mendeleev Communications},
year = {2023},
volume = {34},
publisher = {Mendeleev Communications},
month = {Dec},
url = {https://mendcomm.colab.ws/publications/10.1016/j.mencom.2024.01.005},
number = {1},
pages = {15--17},
doi = {10.1016/j.mencom.2024.01.005}
}
MLA
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Sintsova, Oksana Vladimirovna, et al. “Efficient recombinant production of RpII, a NaV-modulating peptide from the sea anemone Heteractis magnifica.” Mendeleev Communications, vol. 34, no. 1, Dec. 2023, pp. 15-17. https://mendcomm.colab.ws/publications/10.1016/j.mencom.2024.01.005.
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Keywords

acute toxicity
biotechnology
human malignant glioblastoma U87-MG.
NaV
neurotoxin
production in prokaryotes
sea anemone
venom
voltage-gated sodium channels

Abstract

The gene structure and amino acid sequences of the precursor proteins of the sea anemone Heteractis magnifica neurotoxin RpII, a modulator of voltage-gated sodium channels, have been determined. A technology has been developed for the recombinant production of RpII in the Escherichia coli BL21(DE3) expression system based on the pET32b vector containing the thioredoxin gene, which opens up the possibility of obtaining a pure neurotoxin for further structural and/or pharmacological studies. The neurotoxin exhibits a neurotropic effect when administered intravenously to mice in doses greater than 1 mg kg−1, but is not lethal in doses up to 6 mg kg−1 and, in addition, RpII in the nm range does significantly increase the metabolism of human malignant glioblastoma U87-MG.

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