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Fluorescent glycopolymers for probing plant glycan-binding proteins

Alexander Borisovich Tuzikov 1
Alexander Borisovich Tuzikov
, 
Tatiana V Ovchinnikova 1
Tatiana V Ovchinnikova
,  , 
Nicolai Vladimirovich Bovin 1
Nicolai Vladimirovich Bovin
, 
Tatiana A Gorshkova 2
Tatiana A Gorshkova
, 
Tatiana Evgen'evna Chernova 2
Tatiana Evgen'evna Chernova
, 
Nadezhda Vladimirovna Shilova 1
Nadezhda Vladimirovna Shilova
1 M.M. Shemyakin-Yu.A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russian Federation
2 Kazan Institute of Biochemistry and Biophysics, Kazan Scientific Centre of the Russian Academy of Sciences, Kazan, Russian Federation
10.1016/j.mencom.2024.01.004
Published 2023-12-25
Communication , Volume 34, Issue 1, 13-14
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Tuzikov A. B. et al. Fluorescent glycopolymers for probing plant glycan-binding proteins // Mendeleev Communications. 2023. Vol. 34. No. 1. pp. 13-14.
GOST all authors (up to 50) Copy
Tuzikov A. B., Ovchinnikova T. V., Nizovtsev A. V., Bovin N. V., Gorshkova T. A., Chernova T. E., Shilova N. V. Fluorescent glycopolymers for probing plant glycan-binding proteins // Mendeleev Communications. 2023. Vol. 34. No. 1. pp. 13-14.
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TY - JOUR
DO - 10.1016/j.mencom.2024.01.004
UR - https://mendcomm.colab.ws/publications/10.1016/j.mencom.2024.01.004
TI - Fluorescent glycopolymers for probing plant glycan-binding proteins
T2 - Mendeleev Communications
AU - Tuzikov, Alexander Borisovich
AU - Ovchinnikova, Tatiana V
AU - Nizovtsev, Alexey Vadimovich
AU - Bovin, Nicolai Vladimirovich
AU - Gorshkova, Tatiana A
AU - Chernova, Tatiana Evgen'evna
AU - Shilova, Nadezhda Vladimirovna
PY - 2023
DA - 2023/12/25
PB - Mendeleev Communications
SP - 13-14
IS - 1
VL - 34
ER -
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@article{2023_Tuzikov,
author = {Alexander Borisovich Tuzikov and Tatiana V Ovchinnikova and Alexey Vadimovich Nizovtsev and Nicolai Vladimirovich Bovin and Tatiana A Gorshkova and Tatiana Evgen'evna Chernova and Nadezhda Vladimirovna Shilova},
title = {Fluorescent glycopolymers for probing plant glycan-binding proteins},
journal = {Mendeleev Communications},
year = {2023},
volume = {34},
publisher = {Mendeleev Communications},
month = {Dec},
url = {https://mendcomm.colab.ws/publications/10.1016/j.mencom.2024.01.004},
number = {1},
pages = {13--14},
doi = {10.1016/j.mencom.2024.01.004}
}
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Tuzikov, Alexander Borisovich, et al. “Fluorescent glycopolymers for probing plant glycan-binding proteins.” Mendeleev Communications, vol. 34, no. 1, Dec. 2023, pp. 13-14. https://mendcomm.colab.ws/publications/10.1016/j.mencom.2024.01.004.
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Keywords

Cy3
Cy5.
fluorescent probes
glycan-binding proteins
glycopolymers
plant histochemistry

Abstract

The synthesis of glycoprobes based on water-soluble polyacrylamide with a custom content of any low molecular weight fluorescent label is described. The synthesis is carried out in a stepwise manner: in the first step, the polymer is strictly dose-modified with a side substituent-linker containing an amino group; in the second step, the amino group is quantitatively acylated with an activated ester of the dye. The probes are designed to identify and study carbohydrate-binding proteins of plant cells.

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