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X-ray structure of recombinant house dust mite allergen Der p 3

Vladimir Igorevich Timofeev 1
Vladimir Igorevich Timofeev
, 
Yulia Aleksandrovna Abramchik 2
Yulia Aleksandrovna Abramchik
, 
Mikhail B Shevtsov 3
Mikhail B Shevtsov
, 
Maria Andreevna Kostromina 2
Maria Andreevna Kostromina
, 
Sergey Kiriakovich Zavriev 2
Sergey Kiriakovich Zavriev
, 
Evgeny A Zayats 2
Evgeny A Zayats
, 
Roman Stanislavovich Esipov 2
Roman Stanislavovich Esipov
, 
Inna Petrovna Kuranova 1
Inna Petrovna Kuranova
1 A.V. Shubnikov Institute of Crystallography, FSRC ‘Crystallography and Photonics', Russian Academy of Sciences, Moscow, Russian Federaion
2 M.M. Shemyakin-Yu.A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russian Federation
3 Moscow Institute of Physics and Technology (National Research University), Dolgoprudny, Moscow Region, Russian Federation
10.1016/j.mencom.2023.10.019
Published 2023-10-18
Communication , Volume 33, Issue 6, 796-798
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Timofeev V. I. et al. X-ray structure of recombinant house dust mite allergen Der p 3 // Mendeleev Communications. 2023. Vol. 33. No. 6. pp. 796-798.
GOST all authors (up to 50) Copy
Timofeev V. I., Abramchik Y. A., Shevtsov M. B., Kostromina M. A., Zavriev S. K., Zayats E. A., Esipov R. S., Kuranova I. P. X-ray structure of recombinant house dust mite allergen Der p 3 // Mendeleev Communications. 2023. Vol. 33. No. 6. pp. 796-798.
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TY - JOUR
DO - 10.1016/j.mencom.2023.10.019
UR - https://mendcomm.colab.ws/publications/10.1016/j.mencom.2023.10.019
TI - X-ray structure of recombinant house dust mite allergen Der p 3
T2 - Mendeleev Communications
AU - Timofeev, Vladimir Igorevich
AU - Abramchik, Yulia Aleksandrovna
AU - Shevtsov, Mikhail B
AU - Kostromina, Maria Andreevna
AU - Zavriev, Sergey Kiriakovich
AU - Zayats, Evgeny A
AU - Esipov, Roman Stanislavovich
AU - Kuranova, Inna Petrovna
PY - 2023
DA - 2023/10/18
PB - Mendeleev Communications
SP - 796-798
IS - 6
VL - 33
ER -
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@article{2023_Timofeev,
author = {Vladimir Igorevich Timofeev and Yulia Aleksandrovna Abramchik and Mikhail B Shevtsov and Maria Andreevna Kostromina and Sergey Kiriakovich Zavriev and Evgeny A Zayats and Roman Stanislavovich Esipov and Inna Petrovna Kuranova},
title = {X-ray structure of recombinant house dust mite allergen Der p 3},
journal = {Mendeleev Communications},
year = {2023},
volume = {33},
publisher = {Mendeleev Communications},
month = {Oct},
url = {https://mendcomm.colab.ws/publications/10.1016/j.mencom.2023.10.019},
number = {6},
pages = {796--798},
doi = {10.1016/j.mencom.2023.10.019}
}
MLA
Cite this
MLA Copy
Timofeev, Vladimir Igorevich, et al. “X-ray structure of recombinant house dust mite allergen Der p 3.” Mendeleev Communications, vol. 33, no. 6, Oct. 2023, pp. 796-798. https://mendcomm.colab.ws/publications/10.1016/j.mencom.2023.10.019.

Keywords

allergen
crystal structure
Dermatophagoides pteronyssinus
mite
serine protease.
X-ray structure

Abstract

The X-ray structure of the Der p 3 allergen was solved at 2.25 A resolution using the molecular replacement method. The most remarkable difference in conformation of the polypeptide chains between the Der p 3 allergen and bovine trypsin is observed in the disordered parts of the polypeptide chain where the degree of homology is lower. The active site of the Der p 3 allergen which involves the residues of catalytic triad Ser197, His 52, and Asp97 (Ser195, His57, and Asp102 in bovine trypsin) responsible for the specific binding of the positively charged substrates Asp191 (Asp189 in bovine trypsin) is in the cleft between the domains.

References

.
Editorial: Links between allergic rhinitis and asthma still reinforced
Demoly P., Bousquet P.J.
Allergy: European Journal of Allergy and Clinical Immunology, 2008
.
Orchestrating house dust mite-associated allergy in the lung
Gregory L.G., Lloyd C.M.
Trends in Immunology, 2011
.
Activation Mechanism of Recombinant Der p 3 Allergen Zymogen
Dumez M., Teller N., Mercier F., Tanaka T., Vandenberghe I., Vandenbranden M., Devreese B., Luxen A., Frère J., Matagne A., Jacquet A., Galleni M., Chevigné A.
Journal of Biological Chemistry, 2008
.
IgE and IgG anti–house dust mite specificities in allergic disease
Hales B.J., Martin A.C., Pearce L.J., Laing I.A., Hayden C.M., Goldblatt J., Le Souëf P.N., Thomas W.R.
Journal of Allergy and Clinical Immunology, 2006
.
Indoor allergens and asthma:Report of the Third International Workshop
PLATTSMILLS T., VERVLOET D., THOMAS W., AALBERSE R., CHAPMAN M.
Journal of Allergy and Clinical Immunology, 1997
.
Dust Mite Proteolytic Allergens Induce Cytokine Release from Cultured Airway Epithelium
King C., Brennan S., Thompson P.J., Stewart G.A.
Journal of Immunology, 1998
.
Preparation, Crystallization, and Preliminary X-ray Diffraction Analysis of Recombinant House Dust Mite Allergen Der p 3 from Dermatophagoides pteronyssinus
Timofeev V.I., Abramchik Y.A., Zhukhlistova N.E., Mikheeva O.O., Shevtsov M.B., Zayats E.A., Lykoshin D.D., Kostromina M.A., Esipov R.S., Kuranova I.P.
Crystallography Reports, 2023