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Synthesis of disaccharides for the study of human blood antibodies capable of recognizing the inner Glcβ1-3GalNAc disaccharide fragment of bacterial polysaccharides

Galina Valentinovna Pazynina 1
Galina Valentinovna Pazynina
, 
Svetlana Vladimirovna Tsygankova 1
Svetlana Vladimirovna Tsygankova
, 
Polina Sergeevna Obukhova 1, 2
Polina Sergeevna Obukhova
, 
Nadezhda Vladimirovna Shilova 1, 2
Nadezhda Vladimirovna Shilova
, 
Alexander Sergeyevich Paramonov 1
Alexander Sergeyevich Paramonov
, 
Alexander Olegovich Chizhov 3
Alexander Olegovich Chizhov
, 
Yuriy Aleksandrovich Knirel 3
Yuriy Aleksandrovich Knirel
, 
Nicolai Vladimirovich Bovin 1
Nicolai Vladimirovich Bovin
1 M.M. Shemyakin-Yu.A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russian Federation
2 National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V.I. Kulakov, Moscow, Russian Federation
10.1016/j.mencom.2023.06.011
Published 2023-06-13
Communication , Volume 33, Issue 4, 476-478
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Pazynina G. V. et al. Synthesis of disaccharides for the study of human blood antibodies capable of recognizing the inner Glcβ1-3GalNAc disaccharide fragment of bacterial polysaccharides // Mendeleev Communications. 2023. Vol. 33. No. 4. pp. 476-478.
GOST all authors (up to 50) Copy
Pazynina G. V., Tsygankova S. V., Obukhova P. S., Shilova N. V., Paramonov A. S., Chizhov A. O., Knirel Y. A., Bovin N. V. Synthesis of disaccharides for the study of human blood antibodies capable of recognizing the inner Glcβ1-3GalNAc disaccharide fragment of bacterial polysaccharides // Mendeleev Communications. 2023. Vol. 33. No. 4. pp. 476-478.
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TY - JOUR
DO - 10.1016/j.mencom.2023.06.011
UR - https://mendcomm.colab.ws/publications/10.1016/j.mencom.2023.06.011
TI - Synthesis of disaccharides for the study of human blood antibodies capable of recognizing the inner Glcβ1-3GalNAc disaccharide fragment of bacterial polysaccharides
T2 - Mendeleev Communications
AU - Pazynina, Galina Valentinovna
AU - Tsygankova, Svetlana Vladimirovna
AU - Obukhova, Polina Sergeevna
AU - Shilova, Nadezhda Vladimirovna
AU - Paramonov, Alexander Sergeyevich
AU - Chizhov, Alexander Olegovich
AU - Knirel, Yuriy Aleksandrovich
AU - Bovin, Nicolai Vladimirovich
PY - 2023
DA - 2023/06/13
PB - Mendeleev Communications
SP - 476-478
IS - 4
VL - 33
ER -
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@article{2023_Pazynina,
author = {Galina Valentinovna Pazynina and Svetlana Vladimirovna Tsygankova and Polina Sergeevna Obukhova and Nadezhda Vladimirovna Shilova and Alexander Sergeyevich Paramonov and Alexander Olegovich Chizhov and Yuriy Aleksandrovich Knirel and Nicolai Vladimirovich Bovin},
title = {Synthesis of disaccharides for the study of human blood antibodies capable of recognizing the inner Glcβ1-3GalNAc disaccharide fragment of bacterial polysaccharides},
journal = {Mendeleev Communications},
year = {2023},
volume = {33},
publisher = {Mendeleev Communications},
month = {Jun},
url = {https://mendcomm.colab.ws/publications/10.1016/j.mencom.2023.06.011},
number = {4},
pages = {476--478},
doi = {10.1016/j.mencom.2023.06.011}
}
MLA
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Pazynina, Galina Valentinovna, et al. “Synthesis of disaccharides for the study of human blood antibodies capable of recognizing the inner Glcβ1-3GalNAc disaccharide fragment of bacterial polysaccharides.” Mendeleev Communications, vol. 33, no. 4, Jun. 2023, pp. 476-478. https://mendcomm.colab.ws/publications/10.1016/j.mencom.2023.06.011.

Keywords

bacterial polysaccharides
epitope.
glycan synthesis
natural antibodies
printed glycan array

Abstract

Disaccharides with the terminal Glcβ1-3 motif were synthesized as probes for studying human blood antibodies. An antibody isolated using Glcβ1-3GalNAcα-Sepharose was found to bind the inner part of the polysaccharide [-4GlcA6LThr3Acβ1-6Galβ1-6Glcβ1-3GalNAc6Acβ1-]n as evidenced by the use of a printed glycan array and inhibition assays.

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