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Internalization of PLGA nanoparticles coated with poloxamer 188 in glioma cells: A confocal laser scanning microscopy study

Dmitry Vladimirovich Beigulenko 1
Dmitry Vladimirovich Beigulenko
,  , 
Julia Aleksandrovna Malinovskaya 1
Julia Aleksandrovna Malinovskaya
, 
Pavel A Melnikov 2
Pavel A Melnikov
, 
Ekaterina I Medyankina 3
Ekaterina I Medyankina
,  ,  , 
Svetlana Emmanuilovna Gelperina 1
Svetlana Emmanuilovna Gelperina
1 D.Mendeleev University of Chemical Technology of Russia, Moscow, Russian Federation
2 V.P. Serbsky National Medical Research Centre of Psychiatry and Narcology, Moscow, Russian Federation
3 N.I. Pirogov Russian National Research Medical University, Moscow, Russian Federation
10.1016/j.mencom.2023.04.014
Published 2023-04-13
Communication , Volume 33, Issue 3, 340-342
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Beigulenko D. V. et al. Internalization of PLGA nanoparticles coated with poloxamer 188 in glioma cells: A confocal laser scanning microscopy study // Mendeleev Communications. 2023. Vol. 33. No. 3. pp. 340-342.
GOST all authors (up to 50) Copy
Beigulenko D. V., Semyonkin A. S., Malinovskaya J. A., Melnikov P. A., Medyankina E. I., Kovshova T. S., Ermolenko Y. V., Gelperina S. E. Internalization of PLGA nanoparticles coated with poloxamer 188 in glioma cells: A confocal laser scanning microscopy study // Mendeleev Communications. 2023. Vol. 33. No. 3. pp. 340-342.
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TY - JOUR
DO - 10.1016/j.mencom.2023.04.014
UR - https://mendcomm.colab.ws/publications/10.1016/j.mencom.2023.04.014
TI - Internalization of PLGA nanoparticles coated with poloxamer 188 in glioma cells: A confocal laser scanning microscopy study
T2 - Mendeleev Communications
AU - Beigulenko, Dmitry Vladimirovich
AU - Semyonkin, Aleksey Sergeyevich
AU - Malinovskaya, Julia Aleksandrovna
AU - Melnikov, Pavel A
AU - Medyankina, Ekaterina I
AU - Kovshova, Tatyana Sergeevna
AU - Ermolenko, Yulia Valerievna
AU - Gelperina, Svetlana Emmanuilovna
PY - 2023
DA - 2023/04/13
PB - Mendeleev Communications
SP - 340-342
IS - 3
VL - 33
ER -
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@article{2023_Beigulenko,
author = {Dmitry Vladimirovich Beigulenko and Aleksey Sergeyevich Semyonkin and Julia Aleksandrovna Malinovskaya and Pavel A Melnikov and Ekaterina I Medyankina and Tatyana Sergeevna Kovshova and Yulia Valerievna Ermolenko and Svetlana Emmanuilovna Gelperina},
title = {Internalization of PLGA nanoparticles coated with poloxamer 188 in glioma cells: A confocal laser scanning microscopy study},
journal = {Mendeleev Communications},
year = {2023},
volume = {33},
publisher = {Mendeleev Communications},
month = {Apr},
url = {https://mendcomm.colab.ws/publications/10.1016/j.mencom.2023.04.014},
number = {3},
pages = {340--342},
doi = {10.1016/j.mencom.2023.04.014}
}
MLA
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Beigulenko, Dmitry Vladimirovich, et al. “Internalization of PLGA nanoparticles coated with poloxamer 188 in glioma cells: A confocal laser scanning microscopy study.” Mendeleev Communications, vol. 33, no. 3, Apr. 2023, pp. 340-342. https://mendcomm.colab.ws/publications/10.1016/j.mencom.2023.04.014.

Keywords

confocal laser scanning microscopy (CLSM)
GL261 murine glioma cells
poloxamer 188
polylactide-co-glycolide nanoparticles (PLGA NPs)
Rhodamine B.

Abstract

Internalization of poloxamer 188-coated PLGA nanoparticles (NPs) in GL261 murine glioma cells was studied using confocal laser scanning microscopy. For visualization, both poloxamer 188 (P188) and PLGA were labeled covalently with fluorescent dyes Rhodamine B and Cyanine5, respectively. The results indicated that the PLGA NPs coated with poloxamer 188 enter a cell as an integral core-shell structure, which can be helpful for gaining further insight into the in vivo performance of surfactant-coated polymeric NPs as core-shell delivery systems

References

.
Acidic Nanoparticles Are Trafficked to Lysosomes and Restore an Acidic Lysosomal pH and Degradative Function to Compromised ARPE-19 Cells
Baltazar G.C., Guha S., Lu W., Lim J., Boesze-Battaglia K., Laties A.M., Tyagi P., Kompella U.B., Mitchell C.H.
PLoS ONE, 2012
.
Trofimchuk E.S., Moskvina M.A., Ivanova O.A., Potseleev V.V., Demina V.A., Nikonorova N.I., Bakirov A.V., Sedush N.G., Chvalun S.N.
Mendeleev Communications, 2020
.
Drug delivery to the brain using surfactant-coated poly(lactide-co-glycolide) nanoparticles: Influence of the formulation parameters
Gelperina S., Maksimenko O., Khalansky A., Vanchugova L., Shipulo E., Abbasova K., Berdiev R., Wohlfart S., Chepurnova N., Kreuter J.
European Journal of Pharmaceutics and Biopharmaceutics, 2010
.
Delivery of doxorubicin-loaded PLGA nanoparticles into U87 human glioblastoma cells.
Malinovskaya Y., Melnikov P., Baklaushev V., Gabashvili A., Osipova N., Mantrov S., Ermolenko Y., Maksimenko O., Gorshkova M., Balabanyan V., Kreuter J., Gelperina S.
International Journal of Pharmaceutics, 2017
.
Toxicological study of doxorubicin‐loaded PLGA nanoparticles for the treatment of glioblastoma
Pereverzeva E., Treschalin I., Treschalin M., Arantseva D., Ermolenko Y., Kumskova N., Maksimenko O., Balabanyan V., Kreuter J., Gelperina S.
International Journal of Pharmaceutics, 2019
.
Fluorescently Labeled PLGA Nanoparticles for Visualization In Vitro and In Vivo: The Importance of Dye Properties
Zhukova V., Osipova N., Semyonkin A., Malinovskaya J., Melnikov P., Valikhov M., Porozov Y., Solovev Y., Kuliaev P., Zhang E., Sabel B.A., Chekhonin V., Abakumov M., Majouga A., Kreuter J., et. al.
Pharmaceutics, 2021
.
Efficiency of sildenafil encapsulation in poloxamer micelles
Chunhachaichana C., Srichana T.
Journal of Dispersion Science and Technology, 2018
.
In Vitro Displacement by Rat Serum of Adsorbed Radiolabeled Poloxamer and Poloxamine Copolymers from Model and Biodegradable Nanospheres
Neal J.C., Stolnik S., Schacht E., Kenawy E.R., Garnett M.C., Davis S.S., Illum L.
Journal of Pharmaceutical Sciences, 2002
.
Biotin-targeted Pluronic ® P123/F127 mixed micelles delivering niclosamide: A repositioning strategy to treat drug-resistant lung cancer cells
Russo A., Pellosi D.S., Pagliara V., Milone M.R., Pucci B., Caetano W., Hioka N., Budillon A., Ungaro F., Russo G., Quaglia F.
International Journal of Pharmaceutics, 2016
.
Merkulova M.A., Osipova N.S., Maksimenko O.O., Gordienko M.G., Gelperina S.E.
Mendeleev Communications, 2021
.
Colocalization Analysis in Fluorescence Microscopy
Adler J., Parmryd I.
Methods in Molecular Biology, 2012
.
Interaction of surfactant coated PLGA nanoparticles with in vitro human brain-like endothelial cells
Moya E.L., Lombardo S.M., Vandenhaute E., Schneider M., Mysiorek C., Türeli A.E., Kanda T., Shimizu F., Sano Y., Maubon N., Gosselet F., Günday-Türeli N., Dehouck M.
International Journal of Pharmaceutics, 2022