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Optimized method for the recombinant production of a sea anemone’s peptide

Mikhail Nikolaevich Tereshin 1, 2
Mikhail Nikolaevich Tereshin
, 
Alina Mikhailovna Komyakova 1
Alina Mikhailovna Komyakova
, 
Vasily Nikolaevich Stepanenko 1
Vasily Nikolaevich Stepanenko
, 
Igor Valentinovich Myagkikh 1
Igor Valentinovich Myagkikh
, 
Natalia Sergeevna Shoshina 1
Natalia Sergeevna Shoshina
, 
Yuliya Vladimirovna Korolkova 1
Yuliya Vladimirovna Korolkova
, 
Elena Vladimirovna Leychenko 3, 4
Elena Vladimirovna Leychenko
, 
Sergei Aleksandrovich Kozlov 1
Sergei Aleksandrovich Kozlov
10.1016/j.mencom.2022.11.012
Published 2022-10-21
Communication , Volume 32, Issue 6, 745-746
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Tereshin M. N. et al. Optimized method for the recombinant production of a sea anemone’s peptide // Mendeleev Communications. 2022. Vol. 32. No. 6. pp. 745-746.
GOST all authors (up to 50) Copy
Tereshin M. N., Komyakova A. M., Stepanenko V. N., Myagkikh I. V., Shoshina N. S., Korolkova Y. V., Leychenko E. V., Kozlov S. A. Optimized method for the recombinant production of a sea anemone’s peptide // Mendeleev Communications. 2022. Vol. 32. No. 6. pp. 745-746.
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TY - JOUR
DO - 10.1016/j.mencom.2022.11.012
UR - https://mendcomm.colab.ws/publications/10.1016/j.mencom.2022.11.012
TI - Optimized method for the recombinant production of a sea anemone’s peptide
T2 - Mendeleev Communications
AU - Tereshin, Mikhail Nikolaevich
AU - Komyakova, Alina Mikhailovna
AU - Stepanenko, Vasily Nikolaevich
AU - Myagkikh, Igor Valentinovich
AU - Shoshina, Natalia Sergeevna
AU - Korolkova, Yuliya Vladimirovna
AU - Leychenko, Elena Vladimirovna
AU - Kozlov, Sergei Aleksandrovich
PY - 2022
DA - 2022/10/21
PB - Mendeleev Communications
SP - 745-746
IS - 6
VL - 32
ER -
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@article{2022_Tereshin,
author = {Mikhail Nikolaevich Tereshin and Alina Mikhailovna Komyakova and Vasily Nikolaevich Stepanenko and Igor Valentinovich Myagkikh and Natalia Sergeevna Shoshina and Yuliya Vladimirovna Korolkova and Elena Vladimirovna Leychenko and Sergei Aleksandrovich Kozlov},
title = {Optimized method for the recombinant production of a sea anemone’s peptide},
journal = {Mendeleev Communications},
year = {2022},
volume = {32},
publisher = {Mendeleev Communications},
month = {Oct},
url = {https://mendcomm.colab.ws/publications/10.1016/j.mencom.2022.11.012},
number = {6},
pages = {745--746},
doi = {10.1016/j.mencom.2022.11.012}
}
MLA
Cite this
MLA Copy
Tereshin, Mikhail Nikolaevich, et al. “Optimized method for the recombinant production of a sea anemone’s peptide.” Mendeleev Communications, vol. 32, no. 6, Oct. 2022, pp. 745-746. https://mendcomm.colab.ws/publications/10.1016/j.mencom.2022.11.012.

Keywords

analgesic peptide
biotechnology
fluorescence imaging
production in prokaryotes
SUMO fusion
TRPV1 ion channel

Abstract

The production of the analgesic peptide HCRG21 for medical use is restricted by a number of limitations in the technology. The optimal biotechnological method scalable to industrial has been developed based on the production of a fusion protein containing a special leader, His-tag, and Smt3 sequence upstream in the peptide sequence. The resulting peptide shared its inhibitory activity to TRPV1 ion channel identical to the published early.

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