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Comparative structural analysis of a histone-like protein from Spiroplasma melliferum in the crystalline state and in solution

Yury Aleksandrovich Gaponov 1
Yury Aleksandrovich Gaponov
, 
Vladimir Igorevich Timofeev 1, 2
Vladimir Igorevich Timofeev
, 
Yulia Konstantinovna Agapova 1
Yulia Konstantinovna Agapova
, 
Èduard Valeryevich Bocharov 3
Èduard Valeryevich Bocharov
, 
Eleonora Vladimirovna Shtykova 2
Eleonora Vladimirovna Shtykova
, 
Tatiana Vladimirovna Rakitina 1, 3
Tatiana Vladimirovna Rakitina
10.1016/j.mencom.2022.11.011
Published 2022-10-21
Communication , Volume 32, Issue 6, 742-744
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Gaponov Y. A. et al. Comparative structural analysis of a histone-like protein from Spiroplasma melliferum in the crystalline state and in solution // Mendeleev Communications. 2022. Vol. 32. No. 6. pp. 742-744.
GOST all authors (up to 50) Copy
Gaponov Y. A., Timofeev V. I., Agapova Y. K., Bocharov È. V., Shtykova E. V., Rakitina T. V. Comparative structural analysis of a histone-like protein from Spiroplasma melliferum in the crystalline state and in solution // Mendeleev Communications. 2022. Vol. 32. No. 6. pp. 742-744.
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TY - JOUR
DO - 10.1016/j.mencom.2022.11.011
UR - https://mendcomm.colab.ws/publications/10.1016/j.mencom.2022.11.011
TI - Comparative structural analysis of a histone-like protein from Spiroplasma melliferum in the crystalline state and in solution
T2 - Mendeleev Communications
AU - Gaponov, Yury Aleksandrovich
AU - Timofeev, Vladimir Igorevich
AU - Agapova, Yulia Konstantinovna
AU - Bocharov, Èduard Valeryevich
AU - Shtykova, Eleonora Vladimirovna
AU - Rakitina, Tatiana Vladimirovna
PY - 2022
DA - 2022/10/21
PB - Mendeleev Communications
SP - 742-744
IS - 6
VL - 32
ER -
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@article{2022_Gaponov,
author = {Yury Aleksandrovich Gaponov and Vladimir Igorevich Timofeev and Yulia Konstantinovna Agapova and Èduard Valeryevich Bocharov and Eleonora Vladimirovna Shtykova and Tatiana Vladimirovna Rakitina},
title = {Comparative structural analysis of a histone-like protein from Spiroplasma melliferum in the crystalline state and in solution},
journal = {Mendeleev Communications},
year = {2022},
volume = {32},
publisher = {Mendeleev Communications},
month = {Oct},
url = {https://mendcomm.colab.ws/publications/10.1016/j.mencom.2022.11.011},
number = {6},
pages = {742--744},
doi = {10.1016/j.mencom.2022.11.011}
}
MLA
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Gaponov, Yury Aleksandrovich, et al. “Comparative structural analysis of a histone-like protein from Spiroplasma melliferum in the crystalline state and in solution.” Mendeleev Communications, vol. 32, no. 6, Oct. 2022, pp. 742-744. https://mendcomm.colab.ws/publications/10.1016/j.mencom.2022.11.011.

Keywords

crystal structure
histone-like HU protein
NMR
nuclear magnetic resonance spectroscopy
SAXS
small-angle X-ray scattering
structure in solution
X-ray diffraction
XRD

Abstract

A solution of a histone-like protein from Spiroplasma melliferum (HUSpm) was examined by small-angle X-ray scattering (SAXS). The experimental SAXS curve was compared with those calculated for the HUSpm structures from the PDB databank obtained by both X-ray diffraction analysis and nuclear magnetic resonance spectroscopy. The model of the HUSpm structure in solution, which best agrees with the experimental SAXS data, has a shorter distance between the centers of mass of the HUSpm monomers compared to the crystal structure, indicating that the HUSpm monomers can be located closer to each other in solution than in the crystalline state.

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