Keywords
azo coupling
copper(II) complexes
indicator test strip
pyridoxine
solid phase reagent
test method
Abstract
The test method of determination of the pyridoxine (vitamin B6) containing hydroxyaryl group comprises its azo coupling with chromogenic indicator diazo test strip, a reagent with functional diazoaryl group attached to the cellulose carrier through 1,3-diphenoxypropane bridge. This test strip reaction affords cellulose-tethered tetradentate chelating agent capable of forming chromogenic blue-violet complex with CuII ion.
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