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Effect of pH and excitation light wavelength on the activation energy of thermal quenching of tryptophan fluorescence

Sergey Stanislavovich Ostakhov 1
Sergey Stanislavovich Ostakhov
, 
Gul'shat Avkhadievna Masyagutova 1
Gul'shat Avkhadievna Masyagutova
, 
Sergei Leonidovich Khursan 1
Sergei Leonidovich Khursan
10.1016/j.mencom.2020.01.019
Published 2019-12-30
Communication , Volume 30, Issue 1, 59-60
3
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Ostakhov S. S., Masyagutova G. A., Khursan S. L. Effect of pH and excitation light wavelength on the activation energy of thermal quenching of tryptophan fluorescence // Mendeleev Communications. 2019. Vol. 30. No. 1. pp. 59-60.
GOST all authors (up to 50) Copy
Ostakhov S. S., Masyagutova G. A., Khursan S. L. Effect of pH and excitation light wavelength on the activation energy of thermal quenching of tryptophan fluorescence // Mendeleev Communications. 2019. Vol. 30. No. 1. pp. 59-60.
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TY - JOUR
DO - 10.1016/j.mencom.2020.01.019
UR - https://mendcomm.colab.ws/publications/10.1016/j.mencom.2020.01.019
TI - Effect of pH and excitation light wavelength on the activation energy of thermal quenching of tryptophan fluorescence
T2 - Mendeleev Communications
AU - Ostakhov, Sergey Stanislavovich
AU - Masyagutova, Gul'shat Avkhadievna
AU - Khursan, Sergei Leonidovich
PY - 2019
DA - 2019/12/30
PB - Mendeleev Communications
SP - 59-60
IS - 1
VL - 30
ER -
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@article{2019_Ostakhov,
author = {Sergey Stanislavovich Ostakhov and Gul'shat Avkhadievna Masyagutova and Sergei Leonidovich Khursan},
title = {Effect of pH and excitation light wavelength on the activation energy of thermal quenching of tryptophan fluorescence},
journal = {Mendeleev Communications},
year = {2019},
volume = {30},
publisher = {Mendeleev Communications},
month = {Dec},
url = {https://mendcomm.colab.ws/publications/10.1016/j.mencom.2020.01.019},
number = {1},
pages = {59--60},
doi = {10.1016/j.mencom.2020.01.019}
}
MLA
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Ostakhov, Sergey Stanislavovich, et al. “Effect of pH and excitation light wavelength on the activation energy of thermal quenching of tryptophan fluorescence.” Mendeleev Communications, vol. 30, no. 1, Dec. 2019, pp. 59-60. https://mendcomm.colab.ws/publications/10.1016/j.mencom.2020.01.019.

Keywords

activation energy of thermal fluorescence quenching
fluorescence
pH
photoexcitation energy
tryptophan

Abstract

The activation energy of temperature quenching (E) of tryptophan fluorescence at pH 1–13 exhibited a maximum corresponding to the isoelectric point (pHi ≈ 6) of tryptophan at all exciting light wavelengths. The values of E nonmonotonically changed with the excitation wavelength. This behavior was explained by photoinduced electron transfer from the higher singlet-excited Sn (n>1) states of tryptophan.

References

1.
10.1016/j.mencom.2020.01.019_bib0005
Palego
J. Amino Acids, 2016
2.
Ageeva A.A., Babenko S.V., Polyakov N.E., Leshina T.V.
Mendeleev Communications, 2019
3.
Ultraviolet fluorescence of the aromatic amino acids.
Teale F.W., Weber G.
Biochemical Journal, 1957
5.
10.1016/j.mencom.2020.01.019_bib0025
Agarwal
Textbook of Biochemistry, 2007
7.
Photophysics of aqueous tryptophan: pH and temperature effects
Robbins R.J., Fleming G.R., Beddard G.S., Robinson G.W., Thistlethwaite P.J., Woolfe G.J.
Journal of the American Chemical Society, 1980
8.
10.1016/j.mencom.2020.01.019_bib0040
Eisinger
Chem. Phys., 1969
9.
The effect of temperature on the fluorescence of some aromatic amino acids and proteins.
Gally J.A., Edelman G.M.
Biochimica et Biophysica Acta - General Subjects, 1962
10.
Influence of solvent and temperature upon the fluorescence of indole derivatives
Kirby E.P., Steiner R.F.
The Journal of Physical Chemistry, 1970
14.
Anomalous S 2 → S 0 fluorescence of tryptophan complexes with Europium(III) in aqueous ethanol solutions
Ostakhov S.S., Kazakov V.P., Alyab’ev A.S., Osina I.O.
Doklady Physical Chemistry, 2007
16.
10.1016/j.mencom.2020.01.019_bib0080
Bowen
J. Chem. Soc., 1958
17.
10.1016/j.mencom.2020.01.019_bib0085
Jakubke
Aminokisloty, peptidy, belki (Amino Acids, Peptides, Proteins), 1985
18.
Excited state chemistry of aromatic amino acids and related peptides. III. Tryptophan
Bent D.V., Hayon E.
Journal of the American Chemical Society, 1975