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Quantitative characterization of oligomeric state of G-quadruplex antithrombin aptamers by size exclusion HPLC

Rugiya Rufa Alieva 1
Rugiya Rufa Alieva
,  , 
Vadim Neronovich Tashlitsky 1
Vadim Neronovich Tashlitsky
, 
10.1016/j.mencom.2019.07.023
Published 2019-07-01
Communication , Volume 29, Issue 4, 424-425
11
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Alieva R. R. et al. Quantitative characterization of oligomeric state of G-quadruplex antithrombin aptamers by size exclusion HPLC // Mendeleev Communications. 2019. Vol. 29. No. 4. pp. 424-425.
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Alieva R. R., Zavyalova E. G., Tashlitsky V. N., Kopylov A. M. Quantitative characterization of oligomeric state of G-quadruplex antithrombin aptamers by size exclusion HPLC // Mendeleev Communications. 2019. Vol. 29. No. 4. pp. 424-425.
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TY - JOUR
DO - 10.1016/j.mencom.2019.07.023
UR - https://mendcomm.colab.ws/publications/10.1016/j.mencom.2019.07.023
TI - Quantitative characterization of oligomeric state of G-quadruplex antithrombin aptamers by size exclusion HPLC
T2 - Mendeleev Communications
AU - Alieva, Rugiya Rufa
AU - Zavyalova, Elena Gennadievna
AU - Tashlitsky, Vadim Neronovich
AU - Kopylov, Alexey M
PY - 2019
DA - 2019/07/01
PB - Mendeleev Communications
SP - 424-425
IS - 4
VL - 29
ER -
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@article{2019_Alieva,
author = {Rugiya Rufa Alieva and Elena Gennadievna Zavyalova and Vadim Neronovich Tashlitsky and Alexey M Kopylov},
title = {Quantitative characterization of oligomeric state of G-quadruplex antithrombin aptamers by size exclusion HPLC},
journal = {Mendeleev Communications},
year = {2019},
volume = {29},
publisher = {Mendeleev Communications},
month = {Jul},
url = {https://mendcomm.colab.ws/publications/10.1016/j.mencom.2019.07.023},
number = {4},
pages = {424--425},
doi = {10.1016/j.mencom.2019.07.023}
}
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Alieva, Rugiya Rufa, et al. “Quantitative characterization of oligomeric state of G-quadruplex antithrombin aptamers by size exclusion HPLC.” Mendeleev Communications, vol. 29, no. 4, Jul. 2019, pp. 424-425. https://mendcomm.colab.ws/publications/10.1016/j.mencom.2019.07.023.

Abstract

A quantitative technique for estimation of the oligomeric state of G-quadruplexes has been developed for the first time. The simplest 15-mer G-quadruplex antithrombin aptamer HD1 and its analogues, namely RA36 as a covalent dimer of HD1 as well as GL2–HD1 as a non-covalent dimer with G-quadruplex lock, were used as model objects. HD1 and RA36 have been shown to be single-stranded G-quadruplexes, whereas GL2–HD1 forms non-covalent dimers (GL2–HD1)2 in a concentration-dependent manner, affording ∼50% of the dimers at 1mmoldm−3 concentration.

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