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DNA recovery from environmental samples on suspension columns under a combined action of ultrasound and magnetic fields followed by polymerase chain reaction detection

Rustam Kharsanovich Dzhenloda 1
Rustam Kharsanovich Dzhenloda
, 
Dmitriy Grigorievich Petrov 2
Dmitriy Grigorievich Petrov
, 
Valery Mikhailovich Shkinev 1
Valery Mikhailovich Shkinev
, 
Boris Yakovlevich Spivakov 1
Boris Yakovlevich Spivakov
10.1016/j.mencom.2017.05.029
Published 2017-04-25
Communication , Volume 27, Issue 3, 302-303
6
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Dzhenloda R. K. et al. DNA recovery from environmental samples on suspension columns under a combined action of ultrasound and magnetic fields followed by polymerase chain reaction detection // Mendeleev Communications. 2017. Vol. 27. No. 3. pp. 302-303.
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Dzhenloda R. K., Petrov D. G., Shkinev V. M., Spivakov B. Y. DNA recovery from environmental samples on suspension columns under a combined action of ultrasound and magnetic fields followed by polymerase chain reaction detection // Mendeleev Communications. 2017. Vol. 27. No. 3. pp. 302-303.
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TY - JOUR
DO - 10.1016/j.mencom.2017.05.029
UR - https://mendcomm.colab.ws/publications/10.1016/j.mencom.2017.05.029
TI - DNA recovery from environmental samples on suspension columns under a combined action of ultrasound and magnetic fields followed by polymerase chain reaction detection
T2 - Mendeleev Communications
AU - Dzhenloda, Rustam Kharsanovich
AU - Petrov, Dmitriy Grigorievich
AU - Shkinev, Valery Mikhailovich
AU - Spivakov, Boris Yakovlevich
PY - 2017
DA - 2017/04/25
PB - Mendeleev Communications
SP - 302-303
IS - 3
VL - 27
ER -
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@article{2017_Dzhenloda,
author = {Rustam Kharsanovich Dzhenloda and Dmitriy Grigorievich Petrov and Valery Mikhailovich Shkinev and Boris Yakovlevich Spivakov},
title = {DNA recovery from environmental samples on suspension columns under a combined action of ultrasound and magnetic fields followed by polymerase chain reaction detection},
journal = {Mendeleev Communications},
year = {2017},
volume = {27},
publisher = {Mendeleev Communications},
month = {Apr},
url = {https://mendcomm.colab.ws/publications/10.1016/j.mencom.2017.05.029},
number = {3},
pages = {302--303},
doi = {10.1016/j.mencom.2017.05.029}
}
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Dzhenloda, Rustam Kharsanovich, et al. “DNA recovery from environmental samples on suspension columns under a combined action of ultrasound and magnetic fields followed by polymerase chain reaction detection.” Mendeleev Communications, vol. 27, no. 3, Apr. 2017, pp. 302-303. https://mendcomm.colab.ws/publications/10.1016/j.mencom.2017.05.029.

Abstract

A new method is proposed for dynamic DNA extraction from environmental samples (soil, water) on suspension columns containing silica-coated magnetic sorbents (Fe3O4@SiO2) under a combined influence of ultrasound and magnetic fields. The recovered DNA was subsequently determined by real-time polymerase chain reaction on an ANK-32 device. It has been shown for the first time that the suspension columns enable the preconcentration of DNA (taking Mycobacterium tuberculosis as an example) in a dynamic mode from large volumes of water and soil extracts (100 ml and more) to be performed.

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